Preparation of PCR-quality Mouse Genomic DNA With Hot Sodium Hydroxide and Tris (HotSHOT)

Biotechniques. 2000 Jul;29(1):52, 54. doi: 10.2144/00291bm09.

MeSH terms

  • Aging
  • Animals
  • Buffers
  • DNA / analysis
  • DNA / isolation & purification*
  • Deoxyadenine Nucleotides
  • Deoxycytosine Nucleotides
  • Deoxyguanine Nucleotides
  • Hot Temperature*
  • Hydrogen-Ion Concentration
  • Indicators and Reagents
  • Magnesium Chloride
  • Mice
  • Polymerase Chain Reaction*
  • Sodium Hydroxide*
  • Solubility
  • Taq Polymerase
  • Thymine Nucleotides

Substances

  • Buffers
  • Deoxyadenine Nucleotides
  • Deoxycytosine Nucleotides
  • Deoxyguanine Nucleotides
  • Indicators and Reagents
  • Thymine Nucleotides
  • Magnesium Chloride
  • 2'-deoxycytidine 5'-triphosphate
  • Sodium Hydroxide
  • deoxyguanosine triphosphate
  • DNA
  • Taq Polymerase
  • 2'-deoxyadenosine triphosphate
  • thymidine 5'-triphosphate