A novel serological test for the diagnosis of central venous catheter-associated sepsis

J Infect. 2000 May;40(3):262-6. doi: 10.1053/jinf.2000.0656.


Objectives: To determine the sensitivity and specificity of a novel antibody test for the diagnosis of intravascular catheter-related infections due to coagulase-negative staphylococci.

Methods: Sixty-seven patients diagnosed as having central venous catheter (CVC)-associated sepsis based on strict clinical criteria, including positive blood cultures, were compared to 67 patients with a CVC in situ who exhibited no evidence of sepsis. An ELISA serological test based on a novel short-chain lipoteichoic acid antigen isolated from coagulase-negative staphylococci (CNS) was used to determine the patient's serological response (IgG and IgM) to CVC sepsis caused by CNS. The specificity and sensitivity of the test was determined.

Results: There was a significant increase in the antibody levels (IgG and IgM) to the short-chain lipoteichoic acid in patients with CVC-associated staphylococcal sepsis as compared to the control patients.

Conclusions: This new serological method may offer a useful diagnostic test for intravascular catheter infections caused by staphylococci.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Catheterization, Central Venous / adverse effects*
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Female
  • Humans
  • Immunoglobulin G / blood
  • Immunoglobulin M / blood
  • Lipopolysaccharides / immunology
  • Male
  • Middle Aged
  • Sensitivity and Specificity
  • Sepsis / blood
  • Sepsis / diagnosis*
  • Sepsis / etiology
  • Staphylococcal Infections / diagnosis*
  • Staphylococcal Infections / immunology
  • Teichoic Acids / immunology


  • Immunoglobulin G
  • Immunoglobulin M
  • Lipopolysaccharides
  • Teichoic Acids
  • lipoteichoic acid