Molecular manipulation of G-protein-coupled receptors: a new avenue into drug discovery

Curr Med Chem. 2000 Sep;7(9):889-96. doi: 10.2174/0929867003374570.


During the past 10 years or so, associated with the introduction of molecular biology techniques to G protein-coupled receptor (GPCR) research, outstanding progress has been made in understanding the mechanisms of action of these key proteins and their physiological functions. in-vivo manipulation of levels of GPCRs using transgenic and gene knock-out approaches have been particularly successful in assessing the roles of specific GPCRs in animal physiology. Drug discovery is aiming to produce highly specific compounds based on subtle definition of receptor subtypes which can best be studied using heterologous expression of wild type or mutated forms of cDNA or genes encoding these proteins. Furthermore, new therapeutic opportunities may be provided by investigation of orphan receptors, the natural ligands for which remain unidentified. Some human diseases have been shown to be associated with rare mutations of GPCRs and the possibility that widely distributed polymorphisms in GPCR genes may allow selective therapeutic strategies for population subgroups is driving the development of the science of pharmacogenetics.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Drug Design
  • GTP-Binding Proteins / metabolism*
  • Humans
  • Ligands
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Polymorphism, Genetic


  • Ligands
  • Membrane Proteins
  • GTP-Binding Proteins