Membrane permeability modifications are involved in antibiotic resistance in Klebsiella pneumoniae

Biochem Biophys Res Commun. 2000 Aug 2;274(2):496-9. doi: 10.1006/bbrc.2000.3159.

Abstract

Two Klebsiella pneumoniae strains selected according to their high cross-resistance pattern to cephalosporins were characterized by (i) outer membrane protein content such as OmpA or nonspecific porins, (ii) MICs of various cephalosporins and unrelated antibiotics, (iii) beta-lactamase production, and (iv) active efflux of fluoroquinolones. An association of porin deficiency and beta-lactamase production induced a noticeable cephalosporin resistance. In addition to these mechanisms, the presence of an active efflux participating in high-level fluoroquinolone resistance was identified in one strain. The decrease of antibiotic uptake associated with efflux explains the Klebsiella adaptation against the drugs present in the environment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / metabolism
  • Anti-Bacterial Agents / pharmacology*
  • Anti-Infective Agents / metabolism
  • Anti-Infective Agents / pharmacokinetics
  • Anti-Infective Agents / pharmacology*
  • Bacterial Outer Membrane Proteins / analysis
  • Cell Membrane Permeability / drug effects
  • Cell Membrane Permeability / physiology*
  • Drug Resistance, Microbial / physiology
  • Imipenem / pharmacology
  • Klebsiella pneumoniae / chemistry
  • Klebsiella pneumoniae / drug effects*
  • Klebsiella pneumoniae / metabolism*
  • Microbial Sensitivity Tests
  • Norfloxacin / pharmacokinetics
  • beta-Lactamases / metabolism

Substances

  • Anti-Bacterial Agents
  • Anti-Infective Agents
  • Bacterial Outer Membrane Proteins
  • Imipenem
  • beta-Lactamases
  • Norfloxacin