Replicating circular DNA molecules in yeast

Cell. 1975 Mar;4(3):249-53. doi: 10.1016/0092-8674(75)90172-5.


The yeast Saccharomyces cerevisiae contains a class of small circular DNA molecules, approximately 2 mum in contour length (Sinclair et al., 1967). In this report, it is shown that these molecules replicate as double-branched circles, similar to those observed during replication of the bacteriophage lambda and Escherichia coli chromosomes. A normal rate of replication of these DNA circles requires the function of a nuclear gene, cdc 8.

MeSH terms

  • Centrifugation, Density Gradient
  • DNA Replication*
  • DNA, Circular* / biosynthesis
  • Fluorescent Dyes
  • Genes
  • Microscopy, Electron
  • Nucleic Acid Conformation
  • Saccharomyces cerevisiae / metabolism*


  • DNA, Circular
  • Fluorescent Dyes