Regular exercise improves cognitive function and decreases oxidative damage in rat brain

Neurochem Int. 2001 Jan;38(1):17-23. doi: 10.1016/s0197-0186(00)00063-2.


The biochemical mechanisms by which regular exercise significantly benefits health and well being, including improved cognitive function, are not well understood. Four-week-old (young) and 14-month-old (middle aged) Wistar rats were randomly assigned to young control and young exercised, middle-aged control and middle-aged exercised groups. Exercise groups were exposed to a swimming regime of 1 h a day, 5 days a week for 9 weeks. The passive avoidance test showed that middle-aged exercised rats had significantly (P<0.05) better short- (24 h) and long-term (72 h) memory than aged-matched control rats. Conditioned pole-jumping avoidance learning was improved markedly in both age groups by exercise. Brain thiobarbituric acid-reactive substances and 8-hydroxy-2'deoxyguanosine content in the DNA did not change significantly, while the protein carbonyl levels decreased significantly (P<0.05) in both exercised groups. This decrease was accompanied by an increase in the chymotrypsin-like activity of proteasome complex in the exercised groups, whereas trypsin-like activity did not differ significantly between all groups. The DT-diaphorase activity increased significantly (P<0.05) in the brain of young exercised animals. These data show that swimming training improves some cognitive functions in rats, with parallel attenuation of the accumulation of oxidatively damaged proteins.

MeSH terms

  • Age Factors
  • Animals
  • Avoidance Learning / physiology*
  • Brain Chemistry*
  • Citrate (si)-Synthase / metabolism
  • Cognition / physiology*
  • Cysteine Endopeptidases / metabolism
  • Lipid Peroxidation
  • Male
  • Memory / physiology
  • Multienzyme Complexes / metabolism
  • Nerve Tissue Proteins / metabolism
  • Oxidative Stress
  • Physical Conditioning, Animal*
  • Proteasome Endopeptidase Complex
  • Random Allocation
  • Rats
  • Rats, Wistar
  • Swimming
  • Thiobarbituric Acid Reactive Substances / analysis


  • Multienzyme Complexes
  • Nerve Tissue Proteins
  • Thiobarbituric Acid Reactive Substances
  • Citrate (si)-Synthase
  • Cysteine Endopeptidases
  • Proteasome Endopeptidase Complex