Binding of human lipoproteins (low, very low, high density lipoproteins) to recombinant envelope proteins of hepatitis C virus

Med Microbiol Immunol. 2000 Jun;188(4):177-84. doi: 10.1007/s004300000032.


Heterogeneities in the density of hepatitis C virus (HCV)-RNA-carrying material from human sera (1.03-1.20 g/ml) are partially due to the binding of lipoproteins [low density (LDL), very low density (VLDL), high density (HDL) lipoproteins] and immunoglobulins. In this study we demonstrate the binding of recombinant HCV envelope protein (El/E2) to human LDL, VLDL and HDL on a molecular basis. The binding of lipoproteins was restricted to the middle part of the El gene product (amino acids 222-336) and the C-terminal part of the E2 protein (amino acids 523-809). Lipoproteins did not bind to recombinant HCV core protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Baculoviridae
  • Genetic Vectors
  • Hepacivirus / genetics
  • Hepacivirus / metabolism*
  • Humans
  • Lipoproteins, HDL / metabolism*
  • Lipoproteins, LDL / metabolism*
  • Lipoproteins, VLDL / metabolism*
  • Protein Binding
  • Rabbits
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Reticulocytes / metabolism
  • Viral Envelope Proteins / genetics
  • Viral Envelope Proteins / metabolism*


  • E1 protein, Hepatitis C virus
  • Lipoproteins, HDL
  • Lipoproteins, LDL
  • Lipoproteins, VLDL
  • Recombinant Fusion Proteins
  • Viral Envelope Proteins
  • glycoprotein E2, Hepatitis C virus