Radiation-induced apoptosis in the adult central nervous system is p53-dependent

Cell Death Differ. 2000 Aug;7(8):712-20. doi: 10.1038/sj.cdd.4400704.


Oligodendrocytes and subependymal cells in the adult CNS have been shown to undergo radiation-induced apoptosis. Here, we examined the role of p53 in radiation-induced apoptosis in the adult mouse CNS. In the spinal cord of p53+/+ mice, apoptotic glial cells were observed within 24 h after irradiation, and the apoptotic response peaked at 8 h. These apoptotic cells demonstrated the immunohistochemical phenotype of oligodendrocytes, and decreased oligodendrocyte density was observed at 24 h after 22 Gy. A similar time course of radiation-induced apoptosis was seen in subependymal cells in the adult mouse brain. Radiation-induced apoptosis was preceded by an increase in nuclear p53 expression in glial cells of the spinal cord and subependymal cells of the brain. There was no evidence of radiation-induced apoptosis in the spinal cord and subependymal region of p53-/- animals. We conclude that the p53 pathway may be a mechanism through which DNA damage induces apoptosis in the adult CNS.

MeSH terms

  • Animals
  • Apoptosis / radiation effects*
  • Cell Count
  • Central Nervous System / cytology
  • Central Nervous System / radiation effects*
  • Female
  • Immunohistochemistry / methods
  • Mice
  • Mice, Inbred C57BL
  • Neuroglia / cytology
  • Neuroglia / radiation effects
  • Oligodendroglia / cytology
  • Oligodendroglia / radiation effects
  • Rats
  • Rats, Inbred F344
  • Spinal Cord / cytology
  • Spinal Cord / radiation effects
  • Tumor Suppressor Protein p53 / genetics
  • Tumor Suppressor Protein p53 / physiology*


  • Tumor Suppressor Protein p53