Priming of allergic immune responses by repeated ozone exposure in mice

Am J Respir Cell Mol Biol. 2000 Aug;23(2):228-33. doi: 10.1165/ajrcmb.23.2.3898.

Abstract

The effects of repeated ozone exposures on the development of immune responses toward ovalbumin (OVA) were investigated in BALB/c and C57BL/6 mice. Ozone exposures (180 to 500 microg/m(3); 4 h, three times/wk for 4 wk) were combined with a protocol of OVA-aerosol exposure (1% OVA). Immediate cutaneous hypersensitivity (ICHS) reactions and antibody titers were assessed in parallel to cytokine levels of bronchoalveolar lavage fluids. In BALB/c mice, ozone triggered a T-helper (Th)2-like response indicated by dose-dependent increases in total serum immunoglobulin (Ig) E (from 133 to 821 ng/ml), interleukin (IL)-4 (from 60 to 208 pg/ml), and IL-5 levels (from 43 to 356 pg/ml), and by the recruitment of eosinophils and lymphocytes into the airways. Ozone exposure (500 microg/m(3)) in parallel to OVA-aerosol exposure increased anti-OVA IgG(1) antibody titers by 80%, leukotrienes (C(4)/D(4)/E(4)) by 60%, and airway responsiveness (11.3 versus 7.2 mg/ml methacholine), and doubled the frequency of positive ICHS reactions. In C57BL/6 mice, only the combination of OVA and ozone exposure induced positive ICHS reactions, doubled anti-OVA IgG(1), and suppressed anti-OVA IgG(2a) (-64%) antibody titers. Ozone, therefore, shifted the immune responses to OVA toward a Th2-like pattern in both "IgE-high responder" (BALB/c) and "IgE-low responder" (C57BL/6) mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Inhalation
  • Animals
  • Bronchial Hyperreactivity / immunology
  • Bronchoalveolar Lavage Fluid / cytology
  • Cell Count / drug effects
  • Dose-Response Relationship, Drug
  • Female
  • Hypersensitivity, Immediate / immunology*
  • Immunoglobulin E / blood
  • Immunoglobulin E / drug effects
  • Immunoglobulin G / blood
  • Immunoglobulin G / drug effects
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Ovalbumin / immunology
  • Ovalbumin / pharmacology
  • Oxidants, Photochemical / pharmacology*
  • Ozone / pharmacology*
  • Th2 Cells / cytology
  • Th2 Cells / drug effects
  • Th2 Cells / immunology

Substances

  • Immunoglobulin G
  • Oxidants, Photochemical
  • Immunoglobulin E
  • Ozone
  • Ovalbumin