Usefulness of currently available methods for the diagnosis of Anisakis simplex allergy

Allergy. 2000 Jul;55(7):627-33. doi: 10.1034/j.1398-9995.2000.00471.x.


Background: Serodiagnosis of anisakiosis and Anisakis allergy is difficult since many Anisakis antigens show cross-reactivity complications. In the present study, we assess the usefulness of the major immunologic methods currently available for the diagnosis of Anisakis allergy.

Methods: Four tests (skin prick test, CAP-FEIA system, Western blotting, and an antigen-capture ELISA using O-deglycosylated antigen bound by the monoclonal antibody UA3) were applied to Anisakis-free subjects and subjects with confirmed Anisakis allergy.

Results: The skin prick test, CAP-FEIA, and the antigen-capture ELISA identified Anisakis allergy sera with 100% sensitivity, while Western blotting showed 96%/ sensitivity. The antigen-capture ELISA also showed 100% specificity, but CAP-FEIA showed a specificity of only 50%. In Western blotting, none of the bands detected were specific for either Anisakis-free or Anisakis allergy subjects. The skin prick test was not applied to the Anisakis-free subjects, so its specificity could not be determined.

Conclusions: On the basis of these results, we suggest that the most appropriate procedure for diagnosis of Anisakis allergy suspected on clinical grounds is the antigen-capture ELISA using UA3, or, alternatively, a preliminary skin prick test with a positive result subsequently confirmed by UA3-ELISA.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Animals
  • Anisakis / immunology*
  • Antibodies, Helminth / blood
  • Antibodies, Monoclonal / immunology
  • Antigens, Helminth / immunology
  • Blotting, Western / methods*
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Female
  • Humans
  • Hypersensitivity, Immediate / blood
  • Hypersensitivity, Immediate / diagnosis*
  • Hypersensitivity, Immediate / immunology
  • Immunoglobulin E / blood
  • Larva / immunology
  • Male
  • Middle Aged
  • Sensitivity and Specificity
  • Skin Tests / methods*


  • Antibodies, Helminth
  • Antibodies, Monoclonal
  • Antigens, Helminth
  • Immunoglobulin E