Overexpression of T-type calcium channels in HEK-293 cells increases intracellular calcium without affecting cellular proliferation

FEBS Lett. 2000 Jul 28;478(1-2):166-72. doi: 10.1016/s0014-5793(00)01832-9.


Increased expression of low voltage-activated, T-type Ca(2+) channels has been correlated with a variety of cellular events including cell proliferation and cell cycle kinetics. The recent cloning of three genes encoding T-type alpha(1) subunits, alpha(1G), alpha(1H) and alpha(1I), now allows direct assessment of their involvement in mediating cellular proliferation. By overexpressing the human alpha(1G) and alpha(1H) subunits in human embryonic kidney (HEK-293) cells, we describe here that, although T-type channels mediate increases in intracellular Ca(2+) concentrations, there is no significant change in bromodeoxyuridine incorporation and flow cytometric analysis. These results demonstrate that expressions of T-type Ca(2+) channels are not sufficient to modulate cellular proliferation of HEK-293 cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calcium / metabolism*
  • Calcium Channels, T-Type / genetics
  • Calcium Channels, T-Type / metabolism*
  • Cell Cycle* / drug effects
  • Cell Division / drug effects
  • Cell Line
  • DNA / biosynthesis
  • Electric Conductivity
  • Flow Cytometry
  • Humans
  • Hydroxyurea / pharmacology
  • Nocodazole / pharmacology
  • Transfection


  • Calcium Channels, T-Type
  • DNA
  • Nocodazole
  • Calcium
  • Hydroxyurea