Identification of group X secretory phospholipase A(2) as a natural ligand for mouse phospholipase A(2) receptor

FEBS Lett. 2000 Jul 28;478(1-2):187-91. doi: 10.1016/s0014-5793(00)01848-2.


Phospholipase A(2) receptor (PLA(2)R) mediates various biological responses elicited by group IB secretory phospholipase A(2) (sPLA(2)-IB). The recently cloned group X sPLA(2) (sPLA(2)-X) possesses several structural features characteristic of sPLA(2)-IB. Here, we detected a specific binding site of sPLA(2)-X in mouse osteoblastic MC3T3-E(1) cells. Cross-linking experiments demonstrated its molecular weight (180 kDa) to be similar to that of PLA(2)R. In fact, sPLA(2)-X was found to bind the recombinant PLA(2)R expressed in COS-7 cells, and its specific binding detected in mouse lung membranes was abolished by the deficiency of PLA(2)R. These findings demonstrate sPLA(2)-X to be one of the high-affinity ligands for mouse PLA(2)R.

MeSH terms

  • Animals
  • Binding Sites
  • Cell Line
  • Cell Membrane / metabolism
  • Cricetinae
  • Cross-Linking Reagents
  • Group II Phospholipases A2
  • Ligands
  • Lung / cytology
  • Mice
  • Molecular Weight
  • Osteoblasts / cytology
  • Osteoblasts / metabolism
  • Phospholipases A / metabolism*
  • Receptors, Cell Surface / deficiency
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / metabolism*
  • Receptors, Phospholipase A2
  • Recombinant Proteins / metabolism
  • Swine
  • Thermodynamics
  • Transfection


  • Cross-Linking Reagents
  • Ligands
  • Pla2r1 protein, mouse
  • Receptors, Cell Surface
  • Receptors, Phospholipase A2
  • Recombinant Proteins
  • Phospholipases A
  • Group II Phospholipases A2