Antibacterial activity of linoleic and oleic acids isolated from Helichrysum pedunculatum: a plant used during circumcision rites

Fitoterapia. 2000 Aug;71(4):450-2. doi: 10.1016/s0367-326x(00)00150-7.


The antibacterial activity-guided fractionation of the dichloromethane extract of leaves of Helichrysum pedunculatum resulted in the isolation of linoleic and oleic acids. Linoleic acid inhibited the growth of all the Gram-positive bacterial species tested with the minimum inhibitory concentration (MIC) varying between 0.01 and 1.0 mg/ml. Oleic acid was active against three of the five Gram-positive bacteria at a MIC of 1.0 mg/ml. Both compounds were inactive against the Gram-negative species tested. A synergistic effect between the two fatty acids was observed against Staphylococcus aureus and Micrococcus kristinae.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Asteraceae*
  • Circumcision, Male
  • Gram-Negative Bacteria / drug effects*
  • Gram-Positive Bacteria / drug effects*
  • Humans
  • Linoleic Acid / pharmacology*
  • Male
  • Medicine, African Traditional
  • Microbial Sensitivity Tests
  • Oleic Acid / pharmacology*
  • Phytotherapy
  • Plant Extracts / pharmacology
  • Plant Leaves
  • Plants, Medicinal*


  • Anti-Bacterial Agents
  • Plant Extracts
  • Oleic Acid
  • Linoleic Acid