Cloning of the Arabidopsis clock gene TOC1, an autoregulatory response regulator homolog

Science. 2000 Aug 4;289(5480):768-71. doi: 10.1126/science.289.5480.768.

Abstract

The toc1 mutation causes shortened circadian rhythms in light-grown Arabidopsis plants. Here, we report the same toc1 effect in the absence of light input to the clock. We also show that TOC1 controls photoperiodic flowering response through clock function. The TOC1 gene was isolated and found to encode a nuclear protein containing an atypical response regulator receiver domain and two motifs that suggest a role in transcriptional regulation: a basic motif conserved within the CONSTANS family of transcription factors and an acidic domain. TOC1 is itself circadianly regulated and participates in a feedback loop to control its own expression.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Motifs
  • Amino Acid Sequence
  • Arabidopsis / genetics*
  • Arabidopsis / physiology
  • Arabidopsis Proteins*
  • Biological Clocks / genetics*
  • Circadian Rhythm / genetics*
  • Cloning, Molecular
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / physiology
  • Feedback
  • Gene Expression Regulation, Plant
  • Genes, Plant
  • Molecular Sequence Data
  • Mutation, Missense
  • Phenotype
  • Photoperiod
  • Plant Proteins / chemistry
  • Plant Proteins / genetics*
  • Plant Proteins / physiology
  • Plants, Genetically Modified
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • RNA, Plant / genetics
  • RNA, Plant / metabolism
  • Repetitive Sequences, Amino Acid
  • Transcription Factors / chemistry
  • Transcription Factors / genetics
  • Transcription Factors / physiology
  • Transcription, Genetic

Substances

  • Arabidopsis Proteins
  • CONSTANS protein, Arabidopsis
  • DNA-Binding Proteins
  • Plant Proteins
  • RNA, Messenger
  • RNA, Plant
  • TOC1 protein, Arabidopsis
  • Transcription Factors

Associated data

  • GENBANK/AF272039