Production of polyomavirus structural protein VP1 in yeast cells and its interaction with cell structures

FEBS Lett. 2000 Aug 4;478(3):281-9. doi: 10.1016/s0014-5793(00)01787-7.

Abstract

The gene for mouse polyomavirus major structural protein VP1 was expressed in Saccharomyces cerevisiae from the inducible GAL7 promoter. VP1 pseudocapsids were purified from cell lysates. Their subpopulation contained fragments of host DNA, which, in contrast to those of VP1 pseudocapsids produced in insect cells, did not assemble with cellular histones into pseudonucleocores. VP1 pseudocapsids accumulated in the yeast cell nuclei. A strong interaction of VP1 with tubulin fibres of the mitotic spindle was observed. The fibres of spindles were larger in diameter, apparently due to tight VP1 binding. Substantial growth inhibition of yeast cells producing VP1 was observed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Southern
  • Capsid / biosynthesis*
  • Capsid / genetics
  • Capsid / metabolism*
  • Capsid / ultrastructure
  • Capsid Proteins*
  • Cell Division
  • Cell Line
  • Cell Nucleus / chemistry
  • Cell Nucleus / metabolism
  • DNA, Fungal / genetics
  • DNA, Fungal / metabolism
  • Fluorescent Antibody Technique, Indirect
  • Mice
  • Microscopy, Electron
  • Protein Binding
  • Recombinant Proteins / biosynthesis*
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism*
  • Recombinant Proteins / ultrastructure
  • Saccharomyces cerevisiae / cytology*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae / ultrastructure
  • Spindle Apparatus / chemistry
  • Spindle Apparatus / metabolism
  • Spodoptera
  • Tubulin / metabolism

Substances

  • Capsid Proteins
  • DNA, Fungal
  • Recombinant Proteins
  • Tubulin
  • VP1 protein, polyomavirus