Partial Characterization of a Genomic Island Associated With the Multidrug Resistance Region of Salmonella Enterica Typhymurium DT104

FEMS Microbiol Lett. 2000 Aug 15;189(2):285-91. doi: 10.1111/j.1574-6968.2000.tb09245.x.


This study describes the identification of the insertion site and partial characterization of a 43-kb region harboring the genes associated with the penta-resistant phenotype of a Canadian isolate of Salmonella enterica Typhymurium DT104 labelled 96-5227. The 43-kb fragment, here referred to as Salmonella genomic island I (SgiI), was found in the genome of S. enterica Typhymurium between the thdf and a prophage CP-4-like integrase (int2) gene and is flanked by an imperfect 18-bp direct repeat. A region downstream of sulI in the right end of SgiI contained four open reading frames which includes an IS6100 element, and a 2-kb region from the left end contained two open reading frames which showed homology to an integrase and an excisionase. Furthermore, a 1.9-kb retron sequence located between int2 and yidY was identified which may be unique to the S. enterica Typhymurium genome. The int-retron sequence is flanked by a 27-bp imperfect direct repeat.

MeSH terms

  • Base Sequence
  • Drug Resistance, Multiple / genetics*
  • Genes, Bacterial
  • Genome, Bacterial*
  • Molecular Sequence Data
  • Salmonella typhimurium / genetics*