High-performance affinity beads for identifying drug receptors

Nat Biotechnol. 2000 Aug;18(8):877-81. doi: 10.1038/78496.


We have developed a method using novel latex beads for rapid identification of drug receptors using affinity purification. Composed of a glycidylmethacrylate (GMA) and styrene copolymer core with a GMA polymer surface, the beads minimize nonspecific protein binding and maximize purification efficiency. We demonstrated their performance by efficiently purifying FK506-binding protein using FK506-conjugated beads, and found that the amount of material needed was significantly reduced compared with previous methods. Using the latex beads, we identified a redox-related factor, Ref-1, as a target protein of an anti-NF-kappaB drug, E3330, demonstrating the existence of a new class of receptors of anti-NF-kappaB drugs. Our results suggest that the latex beads could provide a tool for the identification and analysis of drug receptors and should therefore be useful in drug development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Benzoquinones / metabolism*
  • DNA Primers
  • Humans
  • Jurkat Cells
  • Molecular Sequence Data
  • NF-kappa B / antagonists & inhibitors
  • Propionates / metabolism*
  • Receptors, Drug / chemistry*
  • Receptors, Drug / metabolism
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism


  • Benzoquinones
  • DNA Primers
  • NF-kappa B
  • Propionates
  • Receptors, Drug
  • Recombinant Proteins
  • E 3330