Structure of the DNA binding domain of E. coli SSB bound to ssDNA

Nat Struct Biol. 2000 Aug;7(8):648-52. doi: 10.1038/77943.

Abstract

The structure of the homotetrameric DNA binding domain of the single stranded DNA binding protein from Escherichia coli (Eco SSB) bound to two 35-mer single stranded DNAs was determined to a resolution of 2.8 A. This structure describes the vast network of interactions that results in the extensive wrapping of single stranded DNA around the SSB tetramer and suggests a structural basis for its various binding modes.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Binding Sites
  • Crystallography, X-Ray
  • DNA, Single-Stranded / chemistry
  • DNA, Single-Stranded / genetics
  • DNA, Single-Stranded / metabolism*
  • DNA-Binding Proteins / chemistry*
  • DNA-Binding Proteins / metabolism*
  • Escherichia coli / chemistry*
  • Models, Molecular
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides / chemistry
  • Oligodeoxyribonucleotides / genetics
  • Oligodeoxyribonucleotides / metabolism
  • Peptide Fragments / chemistry
  • Peptide Fragments / metabolism
  • Protein Binding
  • Protein Structure, Quaternary
  • Spectrometry, Fluorescence
  • Structure-Activity Relationship
  • Thermodynamics

Substances

  • DNA, Single-Stranded
  • DNA-Binding Proteins
  • Oligodeoxyribonucleotides
  • Peptide Fragments

Associated data

  • PDB/1EYG