Particulate matter in reconstituted amphotericin B and assay of filtered solutions of amphotericin B

Am J Hosp Pharm. 1975 Apr;32(4):381-4.

Abstract

Amphotericin B colloidal solutions were prepared according to the manufacturer's directions and filtered with membrane filters of different porosity to test for particulate matter; and the concentration and in vitro microbiological activity of filtered and nonfiltered solutions were determined. The amphotericin B solutions contained numerous particles. Filtration through 0.85- and 0.45-mum filters did not reduce the in vitro antimicrobial activity, and filtration through 5-, 0.45-, and 0.22-mum filters did not alter the concentration of the drug when assayed spectrophotometrically.

MeSH terms

  • Amphotericin B / analysis
  • Amphotericin B / isolation & purification*
  • Amphotericin B / pharmacology
  • Candida albicans / drug effects
  • Colloids
  • Drug Contamination
  • Filtration
  • Particle Size
  • Time Factors

Substances

  • Colloids
  • Amphotericin B