nalyot, a mutation of the Drosophila myb-related Adf1 transcription factor, disrupts synapse formation and olfactory memory

Neuron. 2000 Jul;27(1):145-58. doi: 10.1016/s0896-6273(00)00016-7.

Abstract

nalyot (nal) is a novel olfactory memory mutant of Drosophila, encoding Adf1, a myb-related transcription factor. Following extended training sessions, Adf1 mutants show normal early memory but defective longterm memory. Adf1 shows widespread spatiotemporal expression, yet mutant alleles reveal no discernible disruptions in gross morphology of the nervous system. Studies at the larval neuromuscular junction, however, reveal a role for Adf1 in the modulation of synaptic growth-in contrast to the role established for dCREB2 in the control of synaptic function (Davis et al., 1996). These findings suggest that Adf1 and dCREB2 regulate distinct transcriptional cascades involved in terminal stages of synapse maturation. More generally, Adf1 provides a novel link between molecular mechanisms of developmental and behavioral plasticity.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Activating Transcription Factor 4
  • Alleles
  • Animals
  • Blotting, Northern
  • Blotting, Western
  • Conditioning, Classical / physiology
  • DNA Probes
  • Drosophila
  • Drosophila Proteins*
  • Electrophysiology
  • Embryo, Nonmammalian
  • Gene Expression Regulation / physiology
  • Genes, myb / genetics*
  • Insect Proteins / biosynthesis
  • Insect Proteins / genetics*
  • Larva
  • Leucine Zippers
  • Memory / physiology*
  • Mutation / genetics*
  • Mutation / physiology*
  • Nervous System / growth & development
  • Neuromuscular Junction / physiology
  • Presynaptic Terminals / metabolism
  • Smell / genetics*
  • Smell / physiology*
  • Synapses / physiology*
  • Transcription Factors / biosynthesis
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism

Substances

  • Adf1 protein, Drosophila
  • DNA Probes
  • Drosophila Proteins
  • Insect Proteins
  • Transcription Factors
  • Activating Transcription Factor 4