Thermacetogenium phaeum gen. nov., sp. nov., a strictly anaerobic, thermophilic, syntrophic acetate-oxidizing bacterium

Int J Syst Evol Microbiol. 2000 Jul;50 Pt 4:1601-1609. doi: 10.1099/00207713-50-4-1601.

Abstract

A novel anaerobic, thermophilic, syntrophic acetate-oxidizing bacterium, strain PB(T), was isolated from a thermophilic (55 degrees C) anaerobic methanogenic reactor which had been treating kraft-pulp waste water. The bacterium oxidized acetate in co-culture with a thermophilic hydrogenotrophic methanogen. Strain PB(T), a gram-positive, spore-forming, rod-shaped bacterium grew optimally at 58 degrees C and pH 6.8. The bacterium grew acetogenically on several alcohols, methoxylated aromatics, pyruvate, glycine, cysteine, formate and hydrogen/CO2. Strain PB(T) also oxidized acetate with reduction of sulfate or thiosulfate as the electron acceptor. The bacterium contained MK-7 as the major quinone. The G+C content of the DNA was 53.5 mol%. Comparative 16S rDNA analysis indicated that strain PB(T) belongs to the Bacillus-Clostridium subphylum. However, it was distant from any known genera or micro-organism. The closest known relative was Thermoterrabacterium ferrireducens with 87.4% similarity. The name Thermacetogenium phaeum gen. nov., sp. nov. is proposed. The type strain is strain PBT (= DSM 12270T).

Publication types

  • Comparative Study

MeSH terms

  • Acetates / metabolism*
  • Acetone / metabolism
  • Alcohols / metabolism
  • Anaerobiosis
  • Bacillus
  • Base Composition
  • Benzoquinones
  • Bioreactors / microbiology
  • Carbon Dioxide / metabolism
  • Cloning, Molecular
  • Coculture Techniques
  • Cysteine / metabolism
  • DNA, Bacterial / analysis
  • DNA, Bacterial / chemistry
  • DNA, Ribosomal / analysis
  • Formates / metabolism
  • Glycine / metabolism
  • Gram-Positive Endospore-Forming Bacteria / classification*
  • Gram-Positive Endospore-Forming Bacteria / genetics
  • Gram-Positive Endospore-Forming Bacteria / metabolism
  • Hydrogen / metabolism
  • Hydrogen-Ion Concentration
  • Molecular Sequence Data
  • Oxidation-Reduction
  • Pyruvic Acid / metabolism
  • RNA, Bacterial / genetics
  • RNA, Ribosomal, 16S / genetics
  • Sulfates / metabolism
  • Temperature

Substances

  • Acetates
  • Alcohols
  • Benzoquinones
  • DNA, Bacterial
  • DNA, Ribosomal
  • Formates
  • RNA, Bacterial
  • RNA, Ribosomal, 16S
  • Sulfates
  • formic acid
  • Acetone
  • Carbon Dioxide
  • quinone
  • Hydrogen
  • Pyruvic Acid
  • Cysteine
  • Glycine

Associated data

  • GENBANK/AB020336