CD14+CD16++ cells derived in vitro from peripheral blood monocytes exhibit phenotypic and functional dendritic cell-like characteristics

Eur J Immunol. 2000 Jul;30(7):1872-83. doi: 10.1002/1521-4141(200007)30:7<1872::AID-IMMU1872>3.0.CO;2-2.


We previously reported an increased percentage of CD14+CD16++ monocytes in the peripheral blood of HIV-infected patients but the physiopathological role of this monocyte subset remains unclear. Cells with a CD14+CD16++ phenotype may be obtained in vitro by culturing human peripheral blood monocytes in the presence of GM-CSF, IL-4 and IL-10. In the present study, we compared the phenotypic and functional characteristics of monocytes-derived CD14+CD16++ cells with those of macrophages and dendritic cells. We show that the CD14+CD16++ cells express dendritic cell markers: CD40, CD80, CD86, HLA-DR, CD11b, CD11c, CD18, CD1a, and CD83. Using RNase protection assay, we demonstrate that CD14+CD16++ cell subset expresses a low ratio of IL-1beta/IL-1ra mRNA and expresses IL-6, MIP-1alpha, MIP-1beta, MCP-1, IL-8, RANTES and I-309 transcripts, similar to dendritic cells. CD14+CD16++ cells produce IL-12, MCP-1 and IL-8, as assessed by flow cytometry. Moreover, CD14+CD16++ cells pulsed with different recall antigens induce a potent autologous T cell proliferation. Altogether, these results provide evidence that CD14+CD16++ cells differentiated in vitro from peripheral blood monocytes exhibit dendritic cell characteristics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigen Presentation / immunology*
  • CD40 Antigens / immunology
  • CD40 Ligand
  • Cells, Cultured
  • Cytokines / genetics
  • Dendritic Cells / classification
  • Dendritic Cells / cytology
  • Dendritic Cells / drug effects
  • Dendritic Cells / immunology*
  • Flow Cytometry
  • Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology
  • Humans
  • Immunophenotyping
  • Interleukin-10 / pharmacology
  • Interleukin-12 / biosynthesis
  • Interleukin-4 / pharmacology
  • Kinetics
  • Lipopolysaccharide Receptors / immunology*
  • Membrane Glycoproteins / immunology
  • Monocytes / classification
  • Monocytes / cytology
  • Monocytes / drug effects
  • Monocytes / immunology*
  • RNA, Messenger
  • Receptors, IgG / immunology*
  • Recombinant Proteins


  • CD40 Antigens
  • Cytokines
  • Lipopolysaccharide Receptors
  • Membrane Glycoproteins
  • RNA, Messenger
  • Receptors, IgG
  • Recombinant Proteins
  • Interleukin-10
  • CD40 Ligand
  • Interleukin-12
  • Interleukin-4
  • Granulocyte-Macrophage Colony-Stimulating Factor