Genetic polymorphisms in head and neck cancer risk

Head Neck. 2000 Sep;22(6):609-17. doi: 10.1002/1097-0347(200009)22:6<609::aid-hed10>;2-l.


Objective: To assess whether genetic polymorphisms implicated as risk factors for other tobacco-associated malignancies are associated with altered risk of head and neck squamous cell carcinoma.

Design: Case-control study.

Subjects: One hundred sixty patients with head and neck squamous cell carcinoma recruited from a university-based head and neck oncology clinic and 149 population-based controls.

Methods: Genotyping of the CYP1A1 (Ile462Val), GSTM1 (null), GSTP1 (Ile105Val), GSTT1 (null), and P53 (Arg72Pro) genes was performed by polymerase chain reaction-based techniques on DNA prepared from peripheral blood. In addition, a questionnaire was used to collect demographic information from each subject.

Results: Cases were significantly older (p <.0001) and had significantly greater tobacco use (p <.0001) and were more likely to be male (p <.0001) than were control subjects, thus confirming known risk factors for this disease. When cases and controls were compared by simple chi-square analysis, only the frequency of CYP1A1 (Ile462Val) polymorphism was significantly different between cases and controls (OR =.42; 95% CI =.18-.99; p <.04). However, with a logistic regression model to control for known risk factors, we were unable to demonstrate a significant association with head and neck cancer for any of the polymorphisms tested, including CYP1A1.

Conclusions: This population fails to identify a relationship between the above-mentioned polymorphisms and head and neck cancer.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Carcinoma, Squamous Cell / genetics*
  • Case-Control Studies
  • Cytochrome P-450 CYP1A1 / genetics
  • Female
  • Glutathione S-Transferase pi
  • Glutathione Transferase / genetics
  • Head and Neck Neoplasms / genetics*
  • Humans
  • Isoenzymes / genetics
  • Logistic Models
  • Male
  • Middle Aged
  • Polymerase Chain Reaction
  • Polymorphism, Genetic*
  • Risk Factors
  • Smoking / adverse effects
  • Surveys and Questionnaires
  • Tumor Suppressor Protein p53 / genetics


  • Isoenzymes
  • Tumor Suppressor Protein p53
  • Cytochrome P-450 CYP1A1
  • glutathione S-transferase T1
  • GSTP1 protein, human
  • Glutathione S-Transferase pi
  • Glutathione Transferase
  • glutathione S-transferase M1