Presence of phosphorylcholine on a filarial nematode protein influences immunoglobulin G subclass response to the molecule by an interleukin-10-dependent mechanism

Infect Immun. 2000 Sep;68(9):5466-8. doi: 10.1128/IAI.68.9.5466-5468.2000.


The filarial nematode product ES-62 contains phosphorylcholine (PC) covalently attached to N-linked glycans. ES-62 induced high levels of immunoglobulin G1 (IgG1) antibodies, but no IgG2a, to non-PC epitopes of the molecule following subcutaneous injection into BALB/c mice. Conversely, mice given ES-62 lacking PC demonstrated significant production of both IgG subclasses. Thus, PC appears to block production of IgG2a antibodies to other epitopes on the parasite molecule. A role for interleukin-10 (IL-10) in this effect was shown by the ability of IL-10(-/-) mice to make an IgG2a antibody response to non-PC epitopes of ES-62.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Helminth / blood*
  • Filarioidea / immunology*
  • Immunoglobulin G / blood
  • Immunoglobulin G / classification*
  • Interleukin-10 / physiology*
  • Interleukin-4 / physiology
  • Mice
  • Mice, Inbred BALB C
  • Phosphorylcholine / immunology*


  • Antibodies, Helminth
  • Immunoglobulin G
  • Phosphorylcholine
  • Interleukin-10
  • Interleukin-4