Oral immunization of mice with transgenic tomato fruit expressing respiratory syncytial virus-F protein induces a systemic immune response

Transgenic Res. 2000 Apr;9(2):127-35. doi: 10.1023/a:1008979525909.

Abstract

Respiratory syncytial virus (RSV) is one of the most important pathogens of infancy and early childhood. Here a fruit-based edible subunit vaccine against RSV was developed by expressing the RSV fusion (F) protein gene in transgenic tomato plants. The F-gene was expressed in ripening tomato fruit under the control of the fruit-specific E8 promoter. Oral immunization of mice with ripe transgenic tomato fruits led to the induction of both serum and mucosal RSV-F specific antibodies. The ratio of immunoglobulin subclasses produced in response to immunization suggested that a type 1 T-helper cell immune response was preferentially induced. Serum antibodies showed an increased titer when the immunized mice were exposed to inactivated RSV antigen.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Oral
  • Animals
  • Immunization
  • Immunoglobulin A / blood
  • Immunoglobulin G / blood
  • Lycopersicon esculentum / genetics*
  • Lycopersicon esculentum / metabolism
  • Mice
  • Mice, Inbred BALB C
  • Plants, Genetically Modified
  • Plasmids / genetics
  • Respiratory Syncytial Virus Infections / prevention & control
  • Respiratory Syncytial Virus Vaccines / administration & dosage
  • Respiratory Syncytial Virus Vaccines / immunology*
  • Respiratory Syncytial Viruses / immunology*
  • T-Lymphocytes, Helper-Inducer / immunology
  • Vaccines, Edible / immunology*
  • Vaccines, Subunit / immunology
  • Viral Proteins / biosynthesis
  • Viral Proteins / genetics
  • Viral Proteins / immunology*

Substances

  • Immunoglobulin A
  • Immunoglobulin G
  • Respiratory Syncytial Virus Vaccines
  • Vaccines, Edible
  • Vaccines, Subunit
  • Viral Proteins