LC/MS/MS analyses of an oleander extract for cancer treatment

Anal Chem. 2000 Aug 1;72(15):3547-52. doi: 10.1021/ac991425a.

Abstract

An HPLC/MS/MS method has been developed for the characterization and quantification of the cardiac glycosides oleandrin, odoroside, neritaloside and the aglycone oleandrigenin, all contained in a patented-hot-water extract of Nerium oleander L (Anvirzel). Qualitative analysis of such extracts was achieved using a hybrid tandem quadrupole time-of-flight (QqTOF) mass spectrometer. Collision-induced dissociation (CID) mass spectra of oleandrin, oleandrigenin, odoroside, and neritaloside were obtained with greater than 5 ppm mass accuracy and resolution routinely in excess of 8000 (fwhm). The detection limit for oleandrin of 20 pg (injected) was realized when the precursor-to-product ion transition, m/z 577 --> 373, was monitored. We have also applied the analytical method to the determination of oleandrin, oleandrigenin, neritaloside, and odoroside in human plasma following an intramuscular injection of Anvirzel.

MeSH terms

  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / isolation & purification
  • Antineoplastic Agents / pharmacology*
  • Cardenolides / chemistry
  • Cardenolides / isolation & purification
  • Cardenolides / pharmacology*
  • Cardiac Glycosides / chemistry
  • Cardiac Glycosides / isolation & purification
  • Cardiac Glycosides / pharmacology*
  • Chromatography, High Pressure Liquid / methods
  • Humans
  • Mass Spectrometry / methods
  • Molecular Structure
  • Plant Extracts / chemistry
  • Plants, Medicinal / chemistry

Substances

  • Antineoplastic Agents
  • Cardenolides
  • Cardiac Glycosides
  • Plant Extracts
  • neritaloside
  • odoroside
  • oleandrigenin
  • oleandrin