Serial analysis of gene expression identifies putative metastasis-associated transcripts in colon tumour cell lines

Br J Cancer. 2000 Sep;83(6):725-8. doi: 10.1054/bjoc.2000.1330.


We have used serial analysis of gene expression (SAGE) to identify gene expression differences between a primary colon tumour cell line (SW480) and an isogenic lymph-node metastasis cell line (SW620). Differential expression was confirmed for the following genes: keratin K5, cystatin S, serum amyloid A, the human homologue of yeast ribosomal S28 and the p32 subunit of human pre-mRNA splicing factor SF2. Expression of confirmed differences were also analysed in other metastatic cell lines.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Northern
  • Cell Differentiation
  • Colonic Neoplasms / genetics*
  • Colonic Neoplasms / pathology
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tumor Cells, Cultured