Novel natural products from soil DNA libraries in a streptomycete host

Org Lett. 2000 Aug 10;2(16):2401-4. doi: 10.1021/ol005860z.


As a route to accessing the potential chemical diversity of uncultivable microbes from the soil, combinatorial biosynthetic libraries were constructed by cloning large fragments of DNA isolated from soil into a Streptomyces lividans host. Four novel compounds, terragines A (1), B (2), C (3), and D (4), were isolated from recombinant 436-s4-5b1, and another novel compound, terragine E (5), was isolated from 446-s3-102g1. The structures were determined by a combination of spectroscopic techniques, primarily 2D NMR.

MeSH terms

  • Amides / chemistry
  • Amides / metabolism*
  • Biological Products / chemistry*
  • Biological Products / genetics*
  • Cloning, Molecular
  • DNA, Bacterial / genetics*
  • Gene Library
  • Molecular Structure
  • Soil Microbiology
  • Streptomyces / genetics*
  • Streptomyces / metabolism


  • Amides
  • Biological Products
  • DNA, Bacterial