Development of Polymerase Chain Reaction-Based Assays for Bacterial Gene Detection

J Microbiol Methods. 2000 Aug;41(3):201-9. doi: 10.1016/s0167-7012(00)00160-3.


Polymerase chain reaction-based assays provide rapid, simple, and sensitive detection of bacterial genes, but are not without their drawbacks. This review summarizes the principal advantages and disadvantages of PCR-based bacterial gene detection, provides guidelines for the development and validation of new PCR assays, and describes potential pitfalls that may be encountered and how these can be avoided.

Publication types

  • Review

MeSH terms

  • DNA Primers / chemical synthesis
  • DNA, Bacterial / analysis*
  • Genes, Bacterial / genetics*
  • Polymerase Chain Reaction / methods*
  • Reproducibility of Results
  • Sequence Analysis, DNA


  • DNA Primers
  • DNA, Bacterial