Cathepsin B inhibitory peptides derived from beta-casein

H S Lee; K J Lee

doi:10.1016/s0196-9781(00)00212-6

Cathepsin B inhibitory peptides derived from beta-casein

Peptides. 2000 Jun;21(6):807-9. doi: 10.1016/s0196-9781(00)00212-6.

Authors

H S Lee¹, K J Lee

Affiliation

¹ Department of Microbiology, College of Natural Sciences, and Research Centre for Molecular Microbiology, Seoul National University, 151-742, Seoul, South Korea.

PMID: 10959001
DOI: 10.1016/s0196-9781(00)00212-6

Abstract

Two cathepsin B inhibitory peptides were isolated from a commercial pancreatic digest of casein. The peptides were identified as the Pro-Phe-Pro-Gly-Pro-Ile and the Gly-Pro-Phe-Pro-Ile corresponding to the sequence 61-66 and 203-207 of bovine beta-casein. These peptides showed competitive inhibition for cathepsin B with the K(i) values of 2.31 and 3.30 mM, respectively. Two related analogues, Tyr-Pro-Phe-Pro-Gly-Pro-Ile and Val-Tyr-Pro-Phe-Pro-Gly-Pro-Ile, were synthesized but their cathepsin B inhibitory activity was not detected.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Caseins / pharmacology*
Cathepsin B / antagonists & inhibitors*
Oligopeptides / pharmacology*
Peptide Fragments / pharmacology

Substances

Caseins
Oligopeptides
Peptide Fragments
Cathepsin B