Endothelial cell seeding on crosslinked collagen: effects of crosslinking on endothelial cell proliferation and functional parameters

Thromb Haemost. 2000 Aug;84(2):325-31.


Endothelial cell seeding, a promising method to improve the performance of small-diameter vascular grafts, requires a suitable substrate, such as crosslinked collagen. Commonly used crosslinking agents such as glutaraldehyde and formaldehyde cause, however, cytotoxic reactions and thereby hamper endothelialization of currently available collagen-coated vascular graft materials. The aim of this study was to investigate the effects of an alternative method for crosslinking of collagen, using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide (EDC) in combination with N-hydroxysuccinimide (NHS), on various cellular functions of human umbilical vein endothelial cells (HUVECs) in vitro. Compared to non-crosslinked type I collagen, proliferation of seeded endothelial cells was significantly increased on EDC/NHS-crosslinked collagen. Furthermore, higher cell numbers were found with increasing crosslink densities. Neither the morphology of the cells nor the secretion of prostacyclin (PGI2), von Willebrand factor (vWF), tissue plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI-1) was affected by the crosslink density of the collagen substrate. Therefore, EDC/NHS-crosslinked collagen is candidate substrate for in vivo application such as endothelial cell seeding of collagen-coated vascular grafts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blood Coagulation Factors / drug effects
  • Blood Coagulation Factors / metabolism
  • Cell Adhesion / drug effects
  • Cell Culture Techniques / methods*
  • Cell Division / drug effects
  • Collagen / metabolism
  • Collagen / pharmacology*
  • Cross-Linking Reagents / metabolism
  • Cross-Linking Reagents / pharmacology*
  • Cross-Linking Reagents / standards
  • Endothelium, Vascular / cytology*
  • Endothelium, Vascular / metabolism
  • Epoprostenol / metabolism
  • Ethyldimethylaminopropyl Carbodiimide / metabolism
  • Ethyldimethylaminopropyl Carbodiimide / pharmacology
  • Ethyldimethylaminopropyl Carbodiimide / standards
  • Fibrinolytic Agents / metabolism
  • Humans
  • Microscopy, Electron
  • Plasminogen Activator Inhibitor 1 / metabolism
  • Serine Proteinase Inhibitors / metabolism
  • Succinimides / metabolism
  • Succinimides / pharmacology
  • Succinimides / standards
  • Thymidine / metabolism
  • Time Factors
  • Tissue Plasminogen Activator / drug effects
  • Tissue Plasminogen Activator / metabolism
  • Tritium
  • Umbilical Veins / cytology
  • von Willebrand Factor / metabolism


  • Blood Coagulation Factors
  • Cross-Linking Reagents
  • Fibrinolytic Agents
  • Plasminogen Activator Inhibitor 1
  • Serine Proteinase Inhibitors
  • Succinimides
  • von Willebrand Factor
  • Tritium
  • Collagen
  • Epoprostenol
  • Tissue Plasminogen Activator
  • N-hydroxysuccinimide
  • Ethyldimethylaminopropyl Carbodiimide
  • Thymidine