Molecular responses to changes in the environmental pH are conserved between the fungal pathogens Candida dubliniensis and Candida albicans

Int J Med Microbiol. 2000 Jul;290(3):231-8. doi: 10.1016/S1438-4221(00)80120-4.


In this work we cloned CdPHR1 and CdPHR2 from the human fungal pathogen Candida dubliniensis. The two genes are homologues to the pH-regulated genes PHR1 and PHR2 from Candida albicans. The pH-dependent pattern of expression of CdPHR1 and CdPHR2 was conserved in C. dubliniensis. CdPHR1 could be shown to be functionally equivalent to PHR1. The pH-regulated mode of expression was maintained when CdPHR1 was integrated in C. albicans. This indicates a fundamentally similar mode of expressional regulation in the two species. CdPHR1 was furthermore capable of reversing the aberrant phenotype of a Saccharomyces cerevisiae GAS1 deletion mutant. In this species, however, expression of CdPHR1 was no longer under control of the external pH. Expression of CdPHR1 was not detected when it was introduced into Aspergillus nidulans. In conclusion, C. dubliniensis and C. albicans respond to changes in the environmental pH with a change in cell shape and differential gene expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoenzymes / genetics*
  • Apoenzymes / isolation & purification
  • Apoenzymes / metabolism
  • Aspergillus nidulans / genetics
  • Aspergillus nidulans / metabolism
  • Base Sequence
  • Blotting, Northern
  • Blotting, Southern
  • Candida / genetics*
  • Candida / metabolism
  • Candida albicans / genetics*
  • Candida albicans / metabolism
  • Cloning, Molecular
  • Deoxyribodipyrimidine Photo-Lyase / genetics*
  • Deoxyribodipyrimidine Photo-Lyase / isolation & purification
  • Deoxyribodipyrimidine Photo-Lyase / metabolism
  • Fungal Proteins / genetics*
  • Fungal Proteins / isolation & purification
  • Fungal Proteins / metabolism
  • Gene Deletion
  • Gene Expression Regulation, Fungal
  • Hydrogen-Ion Concentration
  • Membrane Glycoproteins / genetics*
  • Membrane Glycoproteins / isolation & purification
  • Membrane Glycoproteins / metabolism
  • Molecular Sequence Data
  • Phenotype
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins*
  • Sequence Analysis, DNA


  • Apoenzymes
  • Fungal Proteins
  • GAS1 protein, S cerevisiae
  • Membrane Glycoproteins
  • PHR1 protein, Candida albicans
  • Saccharomyces cerevisiae Proteins
  • Deoxyribodipyrimidine Photo-Lyase

Associated data

  • GENBANK/AF184907
  • GENBANK/AF184908