Immunofluorescence of mitotic spindles by using monospecific antibody against bovine brain tubulin

Science. 1975 Mar 14;187(4180):948-50. doi: 10.1126/science.1096300.

Abstract

Monospecific antibody directed against bovine brain tubulin has been purified by affinity chromatography and tested against soluble tubulin and intact microtubules of brain and mitotic apparatus. Binding of the tubulin antibody to the mitotic spindle of rat kangaroo cells was demonstrated in all stages of mitosis by indirect immunofluorescence.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Antibodies*
  • Antigen-Antibody Reactions
  • Brain Chemistry
  • Cattle
  • Cells, Cultured
  • Chromatography, Affinity
  • Electrophoresis, Polyacrylamide Gel
  • Fluorescent Antibody Technique
  • Microtubules / immunology*
  • Mitosis*
  • Nerve Tissue Proteins / immunology*
  • Nerve Tissue Proteins / isolation & purification
  • Organoids*
  • Rabbits / immunology
  • Rats

Substances

  • Antibodies
  • Nerve Tissue Proteins