Antifungal activity of Hinokitiol-related compounds on wood-rotting fungi and their insecticidal activities

Biol Pharm Bull. 2000 Aug;23(8):995-7. doi: 10.1248/bpb.23.995.


Hinokitiol (beta-thujaplicin), beta-dolabrin and gamma-thujaplicin isolated from Thujopsis dolabrata SIEB. et ZUCC var hondai MAKINO showed antifungal activities against all of the wood-rotting fungi examined. The antifungal activity of three compounds on Daedalea dickinsii IFO-4979 was especially strong, their minimum inhibitory concentration (MIC) values being 0.2 microg/ml. Their antifungal activities on D. dickinsii IFO-4979 were as high as that of amphotericin B used as a positive control. Three compounds had strong insecticidal activities on Tyrophagus putrescentiae [50%-lethal concentration (LC50 : g/m2) 0.25 in hinokitiol, 0.02 in beta-dolabrin and gamma-thujaplicin. Their insecticidal activities were higher than that of N,N-diethyl-m-toluamide (DEET, LC50 : 1.46 g/m2) used as a positive control. Three compounds also showed strong insecticidal activity on Coptotermes formosanus [LC50 (g/m2) 0.07 in hinokitiol, 0.05 in beta-dolabrin and gamma-thujaplicin], although their insecticidal activities were much lower than that of commercial chloropyrifos (LC50 : 0.00016 g/m2).

MeSH terms

  • Antifungal Agents / pharmacology*
  • Basidiomycota / drug effects*
  • Insecticides / pharmacology*
  • Monoterpenes*
  • Plants*
  • Tropolone / analogs & derivatives*
  • Tropolone / pharmacology*


  • Antifungal Agents
  • Insecticides
  • Monoterpenes
  • Tropolone
  • beta-thujaplicin