Comparison of inhibitory effects of local anesthetics on immune functions of neutrophils

Int J Immunopharmacol. 2000 Oct;22(10):789-96. doi: 10.1016/s0192-0561(00)00040-0.

Abstract

Immunological effects of a variety of local anesthetics on adhesion, phagocytosis, and the production of superoxide anion and hydrogen peroxide by neutrophils were compared. Neutrophils were isolated by peritoneal lavage from rats, 4 h after injection of 1% glycogen. Lidocaine, mepivacaine, procaine, prilocaine and tetracaine at 1 mg/ml inhibited adhesion, phagocytosis, and the production of superoxide anion and hydrogen peroxide in neutrophils. Moreover, lidocaine, mepivacaine, procaine and prilocaine at 0.1 mg/ml inhibited the production of superoxide anion and hydrogen peroxide but not adhesion or phagocytosis. In contrast, tetracaine at 0.1 mg/ml inhibited phagocytosis, and the production of superoxide anion and hydrogen peroxide but not adhesion. At 0.01 mg/ml, however, tetracaine inhibited the production of superoxide anion and hydrogen peroxide; in contrast, other drugs failed to affect neutrophil function. These results suggest that the local anesthetics may affect adhesion, phagocytosis, and the production of superoxide anion and hydrogen peroxide by neutrophils.

Publication types

  • Comparative Study

MeSH terms

  • Anesthetics, Local / pharmacology*
  • Animals
  • Cell Adhesion / drug effects
  • Cell Survival / drug effects
  • Depression, Chemical
  • Hydrogen Peroxide / metabolism
  • Immunity, Cellular / drug effects*
  • In Vitro Techniques
  • Lidocaine / pharmacology
  • Male
  • Neutrophils / drug effects
  • Neutrophils / immunology*
  • Phagocytosis / drug effects
  • Rats
  • Rats, Wistar
  • Superoxides / metabolism

Substances

  • Anesthetics, Local
  • Superoxides
  • Lidocaine
  • Hydrogen Peroxide