Glufosinate ammonium stimulates nitric oxide production through N-methyl D-aspartate receptors in rat cerebellum

Neurosci Lett. 2000 Sep 1;290(3):209-12. doi: 10.1016/s0304-3940(00)01363-x.

Abstract

Glufosinate ammonium, a structural analogue of glutamate, is an active herbicidal ingredient. The neuronal activities of this compound were investigated by use of a microdialysis system that allowed us to measure nitric oxide production in the rat cerebellum in vivo. Kainate (0.3-30 nmol/10 microliter), N-methyl-D-aspartate (NMDA) (3-300 nmol/10 microliter) and glufosinate ammonium (30-3000 nmol/10 microliter), which were administered through the microdialysis probe at a rate of 1 microliter/min for 10 min, stimulated nitric oxide production. The glufosinate ammonium-elicited increase in nitric oxide production was suppressed by an inhibitor of nitric oxide synthase and was antagonized by NMDA receptor antagonists, but not by a kainate/(+/-)-alphaamino-3-hydroxy-5-methylisoxazole-4-propionic acid receptor antagonist. These results suggest that glufosinate ammonium stimulates nitric oxide production through NMDA receptors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aminobutyrates / toxicity*
  • Animals
  • Cerebellum / cytology
  • Cerebellum / drug effects*
  • Cerebellum / metabolism
  • Glutamic Acid / analogs & derivatives
  • Glutamic Acid / metabolism
  • Herbicides / toxicity*
  • Kainic Acid / pharmacology
  • Male
  • N-Methylaspartate / pharmacology
  • Neurons / drug effects*
  • Neurons / metabolism
  • Nitric Oxide / biosynthesis*
  • Nitric Oxide Synthase / drug effects
  • Nitric Oxide Synthase / metabolism
  • Rats
  • Rats, Inbred F344
  • Receptors, N-Methyl-D-Aspartate / drug effects*
  • Receptors, N-Methyl-D-Aspartate / metabolism

Substances

  • Aminobutyrates
  • Herbicides
  • Receptors, N-Methyl-D-Aspartate
  • Nitric Oxide
  • Glutamic Acid
  • phosphinothricin
  • N-Methylaspartate
  • Nitric Oxide Synthase
  • Kainic Acid