Genomic structure and in vivo expression of the human organic anion transporter 1 (hOAT1) gene

Biochem Biophys Res Commun. 2000 Aug 28;275(2):623-30. doi: 10.1006/bbrc.2000.3230.

Abstract

The human organic anion transporter 1 (hOAT1) plays a key role in the secretion of an array of potentially toxic organic anions including many clinically important drugs. Here we report on the genomic cloning of hOAT1. A human genomic library was used for screening of a PAC (P1 artificial chromosome) clone applying PCR techniques. Sequencing of several restriction subclones and of a PCR-generated clone revealed that the hOAT1 gene spans 8.2 kb and is composed of 10 exons divided by 9 introns. RT-PCR studies in a human kidney specimen led to the detection of two new splice variants, hOAT1-3 and hOAT1-4, showing a 132-bp in-frame deletion. Using fluorescence in situ hybridization (FISH) we mapped the hOAT1 gene as a single signal to chromosome 11q13.1-q13.2. Additionally, 600 bp of the 5' flanking region was analyzed, illustrating the probable transcription start site at nt -280, a NF-kappaB-site at nt -397 and several putative transcription factor binding sites.

MeSH terms

  • Anion Transport Proteins
  • Base Sequence
  • Carrier Proteins / genetics*
  • Chromosome Mapping
  • Chromosomes, Human, Pair 11
  • DNA
  • DNA Primers
  • Exons
  • Humans
  • In Situ Hybridization, Fluorescence
  • Introns
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Anion Transport Proteins
  • Carrier Proteins
  • DNA Primers
  • DNA

Associated data

  • GENBANK/AJ249369
  • GENBANK/AJ251529
  • GENBANK/AJ271205