Abstract
A multivalent, bifunctional flagellum carrying two different adhesive peptides in separate flagellin subunits within a filament was constructed in Escherichia coli. The inserted peptides were the fibronectin-binding 115-mer D repeat region of Staphylococcus aureus and the 302-mer collagen-binding region of YadA of Yersinia enterocolitica. Western blotting, immunoelectron microscopy, and adhesion tests with hybrid flagella from an in trans-complemented DeltafliC E. coli strain showed that individual filaments consisted of both recombinant flagellins.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adhesins, Bacterial / chemistry
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Adhesins, Bacterial / genetics*
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Adhesins, Bacterial / metabolism
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Bacterial Adhesion*
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Biotechnology / methods
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Carrier Proteins / chemistry
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Carrier Proteins / genetics*
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Carrier Proteins / metabolism
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Escherichia coli / genetics
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Escherichia coli / metabolism*
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Flagella / metabolism*
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Flagellin / genetics*
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Flagellin / metabolism
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Peptides / chemistry
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Peptides / genetics
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Peptides / metabolism
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Staphylococcus aureus / chemistry
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Staphylococcus aureus / genetics
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Staphylococcus aureus / metabolism
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Yersinia enterocolitica / chemistry
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Yersinia enterocolitica / genetics
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Yersinia enterocolitica / metabolism*
Substances
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Adhesins, Bacterial
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Bacterial Proteins
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Carrier Proteins
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Peptides
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Recombinant Fusion Proteins
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YadA protein, Yersinia
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fibronectin-binding proteins, bacterial
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Flagellin