The renaissance of fluorescence resonance energy transfer

Nat Struct Biol. 2000 Sep;7(9):730-4. doi: 10.1038/78948.

Abstract

Recent advances in fluorescence resonance energy transfer have led to qualitative and quantitative improvements in the technique, including increased spatial resolution, distance range, and sensitivity. These advances, due largely to new fluorescent dyes, but also to new optical methods and instrumentation, have opened up new biological applications.

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Fluorescence*
  • Fluorescent Dyes / chemistry
  • Fluorescent Dyes / metabolism
  • Humans
  • Metals, Rare Earth / metabolism
  • Models, Molecular
  • Optics and Photonics / instrumentation
  • Protein Conformation
  • Proteins / chemistry*
  • Proteins / metabolism*
  • Sensitivity and Specificity
  • Spectrometry, Fluorescence*

Substances

  • Fluorescent Dyes
  • Metals, Rare Earth
  • Proteins