Total tumor cell elimination with minimum damage to normal tissues in musculoskeletal sarcomas following photodynamic therapy with acridine orange

Oncology. 2000 Aug;59(2):174-80. doi: 10.1159/000012156.


Acridine orange (AO) has unique biological actions enabling tumor visualization (fluorovisualization) and a strong cytocidal effect (photodynamic therapy: AO-PDT) under illumination with blue light. Accordingly, in this study, we attempted to develop a new surgical technique for total tumor cell elimination using these photodynamic reactions with AO in a mouse osteosarcoma model. The results showed that local tumor recurrence was significantly inhibited (23%) in the group treated with curettage under fluorovisualization and AO-PDT, compared to that (80%) in the control group treated with curettage alone under ordinary light. Therefore, we concluded that the combination of curettage under fluorovisualization and AO-PDT may be useful for total tumor cell elimination with minimum damage to normal tissue in musculoskeletal sarcomas.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acridine Orange / therapeutic use*
  • Animals
  • Antineoplastic Agents / therapeutic use*
  • Bone Neoplasms / drug therapy*
  • Curettage
  • Disease Models, Animal
  • Male
  • Mice
  • Mice, Inbred C3H
  • Musculoskeletal Diseases / drug therapy
  • Mutagens / therapeutic use
  • Osteosarcoma / drug therapy*
  • Photochemotherapy*
  • Treatment Outcome


  • Antineoplastic Agents
  • Mutagens
  • Acridine Orange