Spatial distribution of 16S rRNA levels from uncultured acidobacteria in soil

Lett Appl Microbiol. 2000 Aug;31(2):118-22. doi: 10.1046/j.1365-2672.2000.00780.x.

Abstract

The activity of uncultured acidobacteria was monitored in Dutch grassland soils by quantifying their ribosomes. These bacteria were detectable by five different 16S rRNA RT-PCR products in temperature gradient gel electrophoresis fingerprints. The ribosomes in surface soil samples were quantified with multiple competitive RT-PCR along a 1.5-km transect through the grassland. In total, the five members of the acidobacteria were estimated to contribute 4 x 1010 to 1 x 1011 ribosomes g soil-1, representing 7-14% of all bacterial ribosomes. These results indicate that ribosomes from acidobacteria are continuously present and abundant in soil and might contribute significantly to microbial activity in soil.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteria / genetics*
  • Bacteria / growth & development
  • Ecosystem
  • Hydrogen-Ion Concentration
  • Poaceae
  • RNA, Bacterial / genetics
  • RNA, Bacterial / isolation & purification*
  • RNA, Bacterial / metabolism
  • RNA, Ribosomal, 16S / genetics
  • RNA, Ribosomal, 16S / isolation & purification*
  • RNA, Ribosomal, 16S / metabolism
  • Reproducibility of Results
  • Reverse Transcriptase Polymerase Chain Reaction
  • Soil Microbiology*

Substances

  • RNA, Bacterial
  • RNA, Ribosomal, 16S