Expression of various multidrug resistance-associated protein (MRP) homologues in brain microvessel endothelial cells

Brain Res. 2000 Sep 8;876(1-2):148-53. doi: 10.1016/s0006-8993(00)02628-7.


Multidrug resistance-associated protein (MRP) actively transports a broad range of anionic compounds out of the cell. To date, six different homologues of MRP (i.e. MRP1-MRP6) have been identified. The current study examines the expression of the various MRP homologues in both primary cultured bovine brain microvessel endothelial cells (BBMEC) and the capillary-enriched fraction from bovine brain homogenates. RT-PCR analysis demonstrated the presence of MRP1, MRP4, MRP5 and MRP6 in both BBMEC and the capillary-enriched fractions of brain homogenates. While low levels of MRP3 were detected in the BBMEC, it was not observed in the capillary-enriched fraction. In addition, RT-PCR and Western blot studies indicated an absence of MRP2 expression in both blood-brain barrier preparations. The presence of several different MRP homologues in the brain microvessel endothelial cells may be important in controlling the permeability of the blood-brain barrier to organic anions.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • ATP-Binding Cassette Transporters / metabolism*
  • Animals
  • Cattle
  • Cells, Cultured
  • Cerebrovascular Circulation / physiology*
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / metabolism*
  • Humans
  • Hybrid Cells
  • Microcirculation / physiology
  • Multidrug Resistance-Associated Proteins
  • Protein Isoforms / metabolism
  • Rats
  • Tumor Cells, Cultured


  • ATP-Binding Cassette Transporters
  • Multidrug Resistance-Associated Proteins
  • Protein Isoforms