Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor: Formation of a functional heteromeric complex requires interleukin 7 receptor

J Exp Med. 2000 Sep 4;192(5):659-70. doi: 10.1084/jem.192.5.659.

Abstract

The cellular receptor for murine thymic stromal lymphopoietin (TSLP) was detected in a variety of murine, but not human myelomonocytic cell lines by radioligand binding. cDNA clones encoding the receptor were isolated from a murine T helper cell cDNA library. TSLP receptor (TSLPR) is a member of the hematopoietin receptor family. Transfection of TSLPR cDNA resulted in only low affinity binding. Cotransfection of the interleukin 7 (IL-7)Ralpha chain cDNA resulted in conversion to high affinity binding. TSLP did not activate cells from IL-7Ralpha(-/)- mice, but did activate cells from gammac(-/)- mice. Thus, the functional TSLPR requires the IL-7Ralpha chain, but not the gammac chain for signaling.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Chromosome Mapping
  • Cloning, Molecular
  • Cytokines / pharmacology
  • Hematopoiesis / drug effects*
  • Humans
  • Interleukin-7 / pharmacology
  • Lymphocytes / drug effects*
  • Lymphocytes / physiology
  • Male
  • Mice
  • Molecular Sequence Data
  • RNA, Messenger / analysis
  • Receptors, Cytokine / chemistry
  • Receptors, Cytokine / genetics
  • Receptors, Cytokine / physiology*
  • Receptors, Interleukin-7 / chemistry
  • Receptors, Interleukin-7 / physiology*
  • Recombinant Proteins / chemistry
  • Signal Transduction

Substances

  • Cytokines
  • Interleukin-7
  • RNA, Messenger
  • Receptors, Cytokine
  • Receptors, Interleukin-7
  • Recombinant Proteins