Mapping of a new target region of allelic loss at 21q22 in primary gastric cancers

Cancer Lett. 2000 Oct 16;159(1):15-21. doi: 10.1016/s0304-3835(00)00525-5.


To determine the minimal region of deletion on 21q22 in gastric cancer, we performed a high-density loss of heterozygosity (LOH) study with eight polymorphic microsatellite markers. Among the 43 tumors examined, 20 (50%) of 40 informative carcinomas showed LOH at one or more loci. The peak LOH frequency was identified at D21S1820 (34.2%) in 21q22.3. This data suggests that this locus might harbor a new tumor suppressor gene in an area <0.332 Mb in physical map distance defined by D21S1820 and D21S49. Thus, we speculate that trefoil factor family 1 (TFF1), located in this narrow region, might be the most probable candidate gene involved in gastric cancer carcinogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / genetics*
  • Adenocarcinoma / pathology
  • Chromosome Banding
  • Chromosome Mapping
  • Chromosomes, Human, Pair 21 / genetics*
  • DNA, Neoplasm / genetics
  • Humans
  • Loss of Heterozygosity*
  • Microsatellite Repeats
  • Stomach Neoplasms / genetics*
  • Stomach Neoplasms / pathology


  • DNA, Neoplasm