The C. elegans gene lin-9,which acts in an Rb-related pathway, is required for gonadal sheath cell development and encodes a novel protein

Gene. 2000 Aug 22;254(1-2):253-63. doi: 10.1016/s0378-1119(00)00296-1.


The Caenorhabditis elegans gene lin-9 functions in an Rb-related pathway that acts antagonistically to a receptor tyrosine kinase/Ras signal transduction pathway controlling vulval induction. We show that lin-9 is also required for the development of the sheath cells in the hermaphrodite gonad and for the development of the male spicule, rays and gonad. lin-9 is transcribed as two alternatively spliced 2.4kb messages, which use two distinct polyadenylation sites and are SL1 trans-spliced. The conceptual translation of lin-9 cDNA sequences predicts proteins of 642 and 644 amino acids with a significant similarity to predicted Drosophila and vertebrate proteins. We suggest that lin-9 is the founding member of a new protein family that functions in Rb-related pathways in many species.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alternative Splicing
  • Amino Acid Sequence
  • Animals
  • Animals, Genetically Modified
  • Base Sequence
  • Caenorhabditis elegans / cytology
  • Caenorhabditis elegans / genetics*
  • Caenorhabditis elegans Proteins*
  • Cloning, Molecular
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • Disorders of Sex Development
  • Female
  • Genes, Helminth / genetics
  • Genetic Complementation Test
  • Gonads / cytology
  • Gonads / growth & development*
  • Gonads / metabolism
  • Helminth Proteins / genetics*
  • Male
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Phenotype
  • Point Mutation
  • RNA, Helminth / genetics
  • Retinoblastoma Protein / genetics
  • Retinoblastoma Protein / metabolism*
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Transcription, Genetic


  • Caenorhabditis elegans Proteins
  • DNA, Complementary
  • Helminth Proteins
  • LIN-9 protein, C elegans
  • RNA, Helminth
  • Retinoblastoma Protein
  • lin-36 protein, C elegans

Associated data

  • GENBANK/AF269693
  • GENBANK/AF269694