5'-Methoxyhydnocarpin-D and pheophorbide A: Berberis species components that potentiate berberine growth inhibition of resistant Staphylococcus aureus

J Nat Prod. 2000 Aug;63(8):1146-9. doi: 10.1021/np990639k.

Abstract

A new method of bioactivity-directed fractionation, based on multidrug resistant pump (MDR) inhibition in Staphylococcus aureus, was demonstrated. This resulted in the isolation, from berberine-containing Berberis species, of two compounds that are themselves devoid of S. aureus antibacterial activity, but that form potent synergistic couples with a subinhibitory concentration of berberine. The bacterial MDR pump inhibitors were identified as the flavonolignan 2 and the porphyrin 3. Another natural flavonolignan, silybin (8) from Silybum marianum, was also shown to be a bacterial MDR pump inhibitor.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Berberine / pharmacology*
  • Biological Assay
  • Chlorophyll / analogs & derivatives*
  • Chlorophyll / chemistry
  • Chlorophyll / isolation & purification
  • Chlorophyll / pharmacology
  • Chromatography, Gel
  • Chromatography, Thin Layer
  • Colorado
  • Drug Resistance, Microbial
  • Drug Resistance, Multiple*
  • Flavonoids / chemistry
  • Flavonoids / isolation & purification
  • Flavonoids / pharmacology*
  • Gas Chromatography-Mass Spectrometry
  • Magnetic Resonance Spectroscopy
  • Microbial Sensitivity Tests
  • Plant Leaves / chemistry
  • Plants, Medicinal / chemistry*
  • Spectrometry, Mass, Fast Atom Bombardment
  • Spectrophotometry, Ultraviolet
  • Staphylococcus aureus / drug effects*
  • Staphylococcus aureus / growth & development

Substances

  • 5'-methoxyhydnocarpin
  • Flavonoids
  • Berberine
  • Chlorophyll
  • pheophorbide a