Cell cycle-dependent regulation of TIAP/m-survivin expression

Biochim Biophys Acta. 2000 Sep 7;1493(1-2):188-94. doi: 10.1016/s0167-4781(00)00142-1.


TIAP, a murine homologue of human survivin, is a member of the inhibitor of apoptosis (IAP) family and is specifically expressed at G2/M phase of the cell cycle. To elucidate regulatory mechanisms of the cycle-dependent expression, we have analyzed the promoter region of TIAP/mouse survivin (m-survivin). The 5'-flanking region of the TIAP/m-survivin gene contained a TATA-less promoter, two AP2 sites, three NF-kB sites, one Sp1 site, many cell cycle-dependent elements (CDEs) and one cell cycle gene homology region (CHR). Primer extension and 5'-rapid amplification of cDNA ends identified one transcription start site at position -100 upstream of the ATG start site (+1). TIAP/m-survivin promoter-luciferase analysis identified a minimal promoter region within the most proximal -271 bp upstream of the ATG start site, and the region between -410 and -272 was critical for the enhancer activity. The combination between the CHR at -51 and the CDE at -57 is also essential for the cell cycle-dependent expression. Mutation of the CDE/CHR element and the enhancer elements may cause disordered expression of TIAP/m-survivin to affect cell survival and oncogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis
  • Base Sequence
  • Cell Cycle
  • Gene Expression Regulation
  • Gene Library
  • Genes, Reporter
  • Inhibitor of Apoptosis Proteins
  • Mice
  • Microtubule-Associated Proteins*
  • Molecular Sequence Data
  • Neoplasm Proteins
  • Promoter Regions, Genetic
  • Proteins / chemistry
  • Proteins / genetics
  • Proteins / metabolism*
  • Repressor Proteins*
  • Sequence Homology, Nucleic Acid
  • Survivin


  • BIRC5 protein, human
  • Birc5 protein, mouse
  • Inhibitor of Apoptosis Proteins
  • Microtubule-Associated Proteins
  • Neoplasm Proteins
  • Proteins
  • Repressor Proteins
  • Survivin