A novel protein (Fbf-1) that binds to CD95/APO-1/FAS and shows sequence similarity to trichohyalin and plectin

Biochim Biophys Acta. 2000 Sep 7;1493(1-2):249-54. doi: 10.1016/s0167-4781(00)00163-9.


The Fas/Apo-1/CD95 cell surface receptor belongs to the TNF receptor family of cell death inducing molecules. A number of cytosolic adapter proteins that mediate signal transduction of CD95 have been characterized, but some features of the molecular mechanisms of CD95-induced cell death remain elusive. We describe here a novel protein that can interact with the cytosolic domain of the murine CD95 receptor in a yeast two-hybrid assay. This novel protein was termed Fbf-1 for Fas binding factor and bears no sequence similarity to the known CD95 adapter proteins. Fbf-1 is 1173 aa long and has a theoretical molecular weight of around 130 kDa. The protein is expressed in a wide variety of tissues and is localized in the cytoplasm. Fbf-1 is a very hydrophilic protein, highly conserved between mouse and human and bears a carboxyterminal leucine heptad repeat reminiscent of leucine zipper protein interaction domains. In addition, it shows sequence similarity to trichohyalin and plectin pointing to a function as a structural protein.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Amino Acid Sequence
  • Animals
  • Artificial Gene Fusion
  • Carrier Proteins
  • Cytoplasm / chemistry
  • Gene Library
  • Humans
  • Intermediate Filament Proteins / chemistry*
  • Male
  • Mice
  • Molecular Sequence Data
  • Myocardium / metabolism
  • Plectin
  • Point Mutation
  • Protein Isoforms / chemistry
  • Protein Precursors / chemistry*
  • Sequence Homology, Amino Acid
  • Testis / metabolism
  • Transcription Factors / chemistry*
  • Yeasts / genetics
  • fas Receptor / chemistry*


  • Adaptor Proteins, Signal Transducing
  • Carrier Proteins
  • FBF1 protein, human
  • Intermediate Filament Proteins
  • PLEC protein, human
  • Plec protein, mouse
  • Plectin
  • Protein Isoforms
  • Protein Precursors
  • Transcription Factors
  • fas Receptor
  • trichohyalin