The development of a cell array and its combination with laser-scanning cytometry allows a high-throughput analysis of nuclear DNA content

Am J Pathol. 2000 Sep;157(3):723-8. doi: 10.1016/S0002-9440(10)64585-3.


We developed a cell array device for analyzing cellular characteristics such as DNA ploidy, numerical chromosomal aberrations, and antigen expression in multiple specimens in a single experiment. Fifty (10 x 5) spots, 2 mm in diameter, were arrayed in an area of 30 x 16 mm on a glass slide, and approximately 1,000 cells were placed on each spot. To demonstrate the usefulness of the cell array, we measured nuclear DNA content using laser-scanning cytometry for DNA ploidy analysis in nine human tumor cell lines and normal lymphocytes. Combining the cell array with laser-scanning cytometry allows not only measurement of nuclear DNA content for 50 samples but also easy comparison of DNA ploidy among the samples in a single experiment. In addition, we used the cell array for fluorescence in situ hybridization using a DNA probe specific for the pericentromeric region of chromosome 11.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Count
  • Cell Nucleus / genetics*
  • Centromere / genetics
  • Chromosomes, Human, Pair 11
  • DNA, Neoplasm / genetics*
  • Humans
  • Image Cytometry / instrumentation*
  • Image Cytometry / methods
  • In Situ Hybridization, Fluorescence
  • Lymphocytes / cytology
  • Microscopy, Confocal / instrumentation*
  • Microscopy, Confocal / methods
  • Ploidies
  • Tumor Cells, Cultured


  • DNA, Neoplasm