A monoclonal antibody to the DEC-205 endocytosis receptor on human dendritic cells

Hum Immunol. 2000 Aug;61(8):729-38. doi: 10.1016/s0198-8859(00)00144-0.


DEC-205 is a multilectin receptor for adsorptive endocytosis, expressed in mouse dendritic cells (DC) and some epithelia. DEC-205 is homologous to the macrophage mannose receptor (MMR). A cDNA for murine DEC-205 was used to identify 3 overlapping human DEC-205 clones from a lymphocyte library. The human homologue is a transmembrane protein of 1722 aminoacids with 10 externally disposed C-type lectin domains having 77% identity to the mouse counterpart. The NH(2) terminal cysteine-rich and fibronectin type II domains were expressed and used to immunize mice. A hybridoma, MG38, which specifically recognized the immunogen was obtained from a DEC-205 knockout mouse. The antibody precipitated a 205 kD protein from metabolically labeled, monocyte-derived DCs. MG38 labeled mature monocyte-derived DCs but showed weak or no labeling of other peripheral blood mononuclear cells. In tissue sections, MG38 identified DEC-205 on thymic cortical epithelium and DCs in the thymic medulla and tonsillar T cell areas. In contrast, an anti-MMR antibody stained DEC-205 negative, macrophages in the thymus cortex, the trabeculae of the thymus and tonsil, as well as efferent lymphatics in the tonsil. Therefore, the MG38 anti-DEC-205 antibody is useful for identifying DCs and reveals clear differences in sites where MMR and DEC-205 are expressed in lymphoid tissues.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Antigens, CD*
  • Cell Line, Transformed
  • Dendritic Cells / cytology
  • Dendritic Cells / immunology*
  • Endocytosis / immunology*
  • Flow Cytometry
  • Gene Expression
  • Humans
  • Lectins, C-Type*
  • Lipopolysaccharides / immunology
  • Lymphoid Tissue
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / immunology*
  • Membrane Glycoproteins / isolation & purification
  • Mice
  • Mice, Knockout
  • Minor Histocompatibility Antigens
  • Monocytes / cytology
  • Monocytes / immunology
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / immunology*
  • Receptors, Cell Surface / isolation & purification


  • Antibodies, Monoclonal
  • Antigens, CD
  • DEC-205 receptor
  • Lectins, C-Type
  • Lipopolysaccharides
  • Membrane Glycoproteins
  • Minor Histocompatibility Antigens
  • Receptors, Cell Surface